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Laboratory diagnostic of Opportunistic nfections

What are the morphological, physiological and tinctorial features of seraciosis causative agents? Rod-shaped asporogenic capsular or acapsular peritrichs or immobile gram-negative monobacteria of Enterobacteriaceae family, which is chemoorganothrophic and aerobes or facultative anaerobes.
What methods are used for diagnostics of opportunistic infections? The main one is bacteriological Additional are serological and microscopic ones.
What are the pecularities of the microbiologic diagnostics of the opportunistic infections? 1. Often isolate the associations of the microbes. 2. It is needed to detect the number of the conditional pathogenic microorganisms. Critical concentrations of the conditional pathogenic microorganisms is 105-106 in 1 ml. 3. It is needed to detect the pathogenicity of the microbes. 4. It is needed to detect the increasing titer of the specific antibody in the blood.
Etiologic and diagnostic of the bacteriemia and sepsis (labor, wound, urogenital, surgery at all.)   It is very difficult diseases that may be caused by all species microorganisms. For diagnostic it is taking only blood (10-20 ml) and then inoculate its in the 2 bottles with medium for cultivation in aerobic and anaerobic conditions.
Etiology and diagnostics of bronchopulmonary infections: acute and chronic bronchitis, acute and chronic pneumonia, pulmonary abscess   These diseases may be caused by H. influenza, Pneumococcus, Micoplasma and different viruses. The material for researches is smear from the nose or oral cavity, sputum, washing water of the bronchus. At first material staining by Gram and study under light microscope. After that material inoculate on the blood agar, chocolate agar, EYA, Endo medium, then incubate its and study cultures and quantity of the microorganisms.
The etiological criterion of the meaning of the microorganisms isolated from respiratory tract - The number of the S. pneumonia and H. influenzae is more than 106 bacteria in 1 ml. - The number of the conditional pathogenic microorganisms must increase in 2-3 times through 3-5 days.
Etiology, diagnostics of the pus-inflammatory infections: wound, burn, postnatal, postoperative, peritonitis, îsteomyelitis   The causative agents of the pus-inflammatory infections may be Staphylococcus, Streptococcus, Clostidium, Bacteroides, Gram-egative rods et all. The material is examining microscopically and inoculating in the blood agar or sugar broth. After incubation the bacteria growing is studding and the number of microorganism is counting in 1 ml or 1 g of the material.
Etiology, pathogenesis, diagnostics and principles of prophylaxis and medical treatment of urological infections: pyelonephritis, cystitis, urethritis, prostatitis. The causative agents of the urological infections may be Staphylococcus, Streptococcus, Escherichia, Proteus, Pseudomonas, Klebsiella, Mycoplasma. From patients we take 3-5 ml urine and inoculate it in the media. The etiological criterion of the meaning of the microorganisms isolated from urinary tract is 105 bacteria in 1 ml of urine.
Etiology, pathogenesis, diagnostics and principles of prophylaxis and medical treatment of acute intestinal infections: food poison and food intoxications The causative agents of the acute intestinal infections are Staphylococcus, Clostridium botulinum, Salmonella, Pseudomonas, Vibrio During studying the result of the bacteriological investigation you must pay attention to species structure and quantitative correlation between different species.
       

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Date: 2016-01-14; view: 1118


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LABORATORY DIAGNOSTIC OF THE OPORTUNISTIC INFECTIONS | QUESTIONS FOR DISCUSSION
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