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Task 9. Differentiating features of bordetella.

Features B. pertussis B. parapertussis B. bronchiseptica B. avium
Motiliti - - + +
Growth on MacConkey agar - - - +
Growth on Borde-Gengou medium (days) 3 - 6 1 - 2
Urease - + + -
Nitrate to nitrite - - + -
Citrate utilization - Variable + Variable
Oxidase + - + +
Heat labile toxin and tracheal cytotoxin + + + +
Adenylate cyclase toxin + + + -
Pertussis toxin + - - -

Task.10 The agglutination test with patient's pair sera and whooping cough diagnosticum.

Ingredients Number of the test tube
0.9 % NaCl solution, ml 1.0 1.0 1.0 1.0 1.0 1.0
Serum dilution 1:40 1:80 1:160 1:320 1:640 -
Diagnosticum, ml 0.1 0.1 0.1 0.1 0.1 0.1
Incubation at 37°C for 1 hour, then at room temperature for 18 hours
Reading 1st serum            
2nd serum            

Note. Reaction is performed simultaneously with pair patient‘s sera.

Task 11.

APDT is vaccine ądsorbed on the hydrate oxide of aluminium, that conteins of the killed whooping-cough bacteria and diphtherial and tetanic toxoids. It is used for planned prophylaxis.

ADTis ądsorbed toxoid of diphtheria and tetanus causative agents; utillized for planned revaccination.

ADT-M is preparation with the diminished amount of antigens, which is utillized for persons with violation of immune status.

Diphtiriae toxoid (DT) is monovaccine, which can be utillized both for the planned vaccination of the chidren and on epidemiology testimonies. It is defuzed at temperature 37°Ń and by formalin 0,4% exotoxin of Corynebacterium diphtheriae.

Antidiphterial antitoxic serum is got by hyperimmunization of horse, a diphtherial toxoid. Effectiveness is for specific therapy of diphtheria. The rules of heterogenic serum introduction should be followed to prevent the development of anaphylactic shock and serum illness.

Antidiphterial gamma-globulin contains antitoxins which are able to neutralize diphtherial exotoxin. It is gamma-globulin fraction of blood serum of the hyperimmunized animals. It is a diminished amount of ballast matters.

Schick test

This procedure has been used during outbreaks of diphtheria to determine which case contacts are susceptible to the disaese, and thus in need of immunization. A small amount of diphtheria toxin is injected intradermally into one forearm and a heated toxin control into the other. Redness and induration within 1 to 2 days at the test site only, or their appearance at the both sites with persistence at the test site but disappearance from the control site in 4-7 days, signifies susceptibility due to lack of sufficient antitoxin. In contrast, no reaction at both the test and control sites, or redness at both sites within 1 day, reaching a maximum in 2-3 days, and fading rapidly from both sites, signifies immunity due to the presence of sufficient neutralizing antitoxin.

Addition 1

Date: 2016-01-14; view: 1512

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Task 4. The differentiating properties of corynebacteria | Laboratory Diagnostics of the Diphtheriae and Pertussis
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