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QUESTIONS FOR DISCUSSION1. General description of the Enterobacteriaceae family bacteria. 2. Biological properties of E. coli, antigen structure and classification of pathogenic intestinal rods. 3. Microbiological diagnostics of Escherichiosis: diarrhoeal Escherichiosis and coli-bacteriosis. 4. Epidemiology and pathogenesis of diarrhoeal Escherichiosis. Specific features of immunity in such cases of Escherichiosis. 5. Principles of prophylaxis and medical treatment of such type of diarrhea. PROCEDURE OF PRACTICAL SESSION Task 1. Study the preparation from the pure cultures of E. coli O1: K1”and ”E. coli O55: K59:H1”, microscopically; draw them in the protocol. Task 2. Study cultural properties of causative agents on Endo and EMB agar: to sketch colonies, to make conclusions and to mark the plan of the further researches. Task 3. Study the antigenic structure of Escherichia and estimate the reaction of agglutination on glass with Escherichiosis polyvalent agglutination OK- and monovalent group sera. Task 4. Study the growth of E. coli on the Triple sugar iron (TSI) agar. Task 5. Study the biochemical properties of E. coli on the Hiss mideum. Task 6. Study the sensitivity of E. coli to antibacterial preparations. Task 7. Study the main antimicrobial drugs used for treatment, prevention and diagnostics of suppurative diseases. Write them in your copybook. RECOMMENDATIONS FOR PRACTICAL WORK Task 1.
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Task 7. Agglutinating escherichiosis polivalent OKA-serumisdiagnosticpreparation, which contains specific antibody to the superficial (Ê-) and somatic (Î-) antigens of E. coli, which together with the proper antigens of E. coli cause their agglutination. Preparation is obtained from the serum of the rabbit, hyperimmunized by the mixture of corpuscular antigens of E. coli (O18ac:K84, O23: K11, O75:Ê_ and other). Preparation is used for the raising of reaction of agglutination on glass with the target of reference determination of the serogroup of pathogenic E. coli. Preparation is canned by chloroform, packed up and lyophilized. At the reaction of agglutination it is necessary to make the working solution of the serum 1:10. Agglutinating adsorbed esherichiosis monovalency serum O55. isdiagnosticpreparation, which contains specific antibody to the somatic (O55) antigen of E. coli, which together with antigen O55 of E. coli causes their agglutination. Preparation is obtained from the serum of the rabbit, hyperimmunized by a corpuscular O55 antigen of E. coli with the subsequent adsorption of unspecific antibodies. Preparation is used for the raising of reaction of agglutination (with the warmed culture) to determine O55 antigen of E. coli, the serogroup of E. coli. Coli-bacterinis treatment and prophylactic preparation,which contains lyophilical cells of the E. coli culture of M17, which express antagonistic characteristics in relation to the definite pathogenic intestinal bacteria. Preparation is used for prevention and treatment of the dysbacteriosis at children. Coli-bacterin is administered to children from 6-month age. While using this remedy the simultaneous application of vitamins is necessary. Bifidumbacterinis treatment and prophylactic preparationwhich contains the lyophilical cells Bifidobacterium bifidum is used for prevention and treatment of the dysbacteriosis in children. Bificolis therapeutic and prophylactic preparation which contains lyophilical cells of the E. coli culture M17 and B. bifidum is used for normalization of the intestinal microflora at dysbacteriosis. Lactobacterinis therapeutic preparation,contains obligate for the man milky-sour bacteria and products of their vital activity, which show antagonistic activity in relation to the pathogenic and to the conditional-pathogenic enterobacteria. It is produced in the form of tablets used for the medical treatment of dysbacteriosis at children. Addition 1 Date: 2016-01-14; view: 2101
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