AFFECT OF P53 GENE MUTATION ON SOME PATHOMORPHOLOGICAL PARAMETERS OF RECTAL CARCINOMA

R.B. BAYRAMOV, V.J. MAHMUDOV, R.T. ABDULLAEVA

Azerbaijan Medical University, Baku, AZERBAIJAN

P53 gene mutation plays one of the definitive roles in the development of colorectal carcinoma. In cells where p53 gene mutation developed the p53 protein truncation occurs, and the latter can be detected immunohistochemically. The aim of the trial is to study the effect of p53 gene mutation on mitotic index of tumor cells, extent of necrosis in tumor tissue, gradation of tumor and mucin–producing activity of tumor cells in cases of rectal carcinoma. In order to reach the aim we studied the p53 protein truncation in 61 patients with rectal carcinoma who underwent radical surgery by our surgical team in Department of Oncology, Azerbaijan Medical University from January 2001 to December 2009. During this period of time 176 patients were operated in the above-indicated institute. Retrospectively the paraffin-embedded tumor tissue materials were intentionally collected in all patients and given to special immunohistochemical investigation. Only in 61 of 179 patients the tumor tissue materials were accepted fit for this investigation. The immunohistochemical investigation was fulfilled by policlonal antibody against truncuated APC protein (BIOZOL, USA) according to generally accepted guidelines. We tried to study parallels between p53 gene mutation in rectal carcinoma cells and mitotic index of tumor cells, extent of necrosis in tumor tissue, gradation of tumor and mucin–producing activity of tumor cells. There was found no correlation between p53 protein truncation and the first three parameters. Statistical significant correlation was revealed between p53 protein truncation and mucin–producing activity of rectal carcinoma cells. So in 19 (82.6%) of 23 cases of rectal carcinoma without p53 protein truncation no amount of mucin was found in carcinoma cells. Mucin filled the ≤30% of cytoplasma in 8 (21.0), >30% in 11 cases (29.0%) with p53 protein truncation (p<0.05). So the facts confirm the direct or indirect affect of p53 gene mutation on mucin–producing activity of rectal carcinoma cells. May be it is related to close locations of p53 gene and the responsible gene for mucin production. The other probable mechanism may be due to suppressive role of p53 gene on mucin production. Further investigations are needed to study its mechanism and significance.

Date: 2014-12-28; view: 921