Creating a synthetic genome

In 2008, scientists put genome from one organism into cell of another, taking over cell’s machinery.

These were grown up in a bacterium, and knitted together into bigger pieces, so-called “cassettes” of genes.

The researchers ended up with several large chunks of DNA that were joined to make the circular genome of a synthetic version of Mycoplasma genitalium.

They have named it Mycoplasma JCVI-1.0, after their research centre, the J Craig Venter Institute in Rockville, MD, US.

Dr Craig Venter, who was involved in the race to decode the human genome, believes tailor-made micro-organisms can become efficient producers of non-polluting fuels such as hydrogen. Other synthetic bacteria could be made to take up greenhouse gases, he believes.

“It sets the stage for what we hope is going to be a new approach to engineering organisms,” said co-researcher Dr Smith.

Operating systems

To achieve this goal, the researchers must overcome a crucial, and tricky obstacle. They must transplant the synthetic genome into another cell so that it can use the existing machinery to “boot up” and start growing and reproducing.

“It’s installing the software - basically we have to boot up the genome, get it operating,” said Dr Smith, who shared a Nobel Prize in 1978 for furthering knowledge on how to cut up segments of DNA.

“We’re simply re-writing the operating software for cells - we’re not designing a genome from the bottom up - you can’t drop a genome into a test tube and expect it to come to life,” he added.

This is the stage which raises the most concern among critics, and where a new life form could be said to be truly created. How precisely will it behave? What will its impact be on other organisms and the environment? Some say it is a step too far, but others argue that the new field of synthetic biology is an important science.

Even bigger

The UK’s Royal Society is seeking views from the public on the issue. Adviser on synthetic biology, Dr Jason Chin, said the increasing ability to design and construct DNA sequences would, in principle, allow the construction of organisms for particular purposes, such as biofuels production.

He added: “Understanding how you construct organisms artificially is an important first step. But scientists still need to understand what effect altering the DNA sequence of an organism - such as bacteria - will have upon their behaviour.”

Dr Drew Endy of the Department of Biological Engineering at Massachusetts Institute of Technology, US, said that reconstructing a natural bacterial genome from scratch was a great technical feat.

He said genomes 10 times larger than Mycoplasma JCVI-1.0 had already been assembled from existing DNA fragments by a Japanese group.

Dr Endy added: “Given the work already done in Japan, building genomes almost 10 million base-pairs long - I would be surprised if by 2012 it were not technically possible to routinely design and construct the genomes of any bacteria or single celled eukaryote, which also means that it will be possible to construct some mammalian chromosomes.”

Dr Simon Woods, a bio-ethicist at the Policy, Ethics and Life Sciences Research Centre at the University of Newcastle, UK, said scientists were acting in a regulatory vacuum.

“On the one hand it’s an amazing piece of science but the real concern is that it’s another example of science delving into matters that have potentially dangerous consequences. It’s not necessarily going to stay in the hands of well-intentioned scientists,” he said.

Answer the following questions:

1. What did the team of 17 scientists construct?

2. Why does the group hope to use engineered genomes?

3. What did co-researcher Dr Smith say?

4. What would the increasing ability to design and construct DNA sequences allow?

5. What did Dr Simon Woods say?

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Date: 2016-04-22; view: 970