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Microbiological research of the Epidemic relapsing fever


 

Microbiological research of the leptospirosis

 

ALGORITHM OF ACTIONS

DURING CONDUCTION OF MICROBIOLOGICAL DIAGNOSIS OF SYPHILIS

T. pallidum immobilization tests (TPT) – Dilution of the serum are mixed with complement and with live, actively motile T.pallidum from the testicular chancre of the rabbit, and the mixture is observed microscopically. If specific antibodies are present, spirochetes are immobilized; in normal serum active motion continues.

 

LABORATORY DIAGNOSTICS OF RICKETTSIA DISEASES

Actuality. Rickettsia diseases are caused by small bacteria that are obligate intracellular parasites and – except for Q fever – are transmitted in humans by arthropods.

Primary objectives: to be able to conduct and evaluate the microbiological diagnostics of Rickettsia diseases.

QUESTIONS FOR DISCASSION

1. General description of bacteria of the family Rickettsia.

2. Microbiological diagnosis of Rickettsia diseases.

PROCEDURE OF PRACTICAL SESSION

Task 1. To do and estimate hemagglutination test.

Task 2. Study the smears from patient’s materials and draw it’s in protocol.

Task 3. Discrybe the immunobiological preparates for diagnostic, treatment and prophylaxis of spirochetosis.

RECOMMENDATIONS FOR PRACTICAL WORK

Task 1. The main method of Rickettsia diseases diagnosis is serological. This test is making with patient's pair sera. There is increasing in four times of titer of antibody in second serum (that serum is taking from patients after 14 days from beginning of diseased). It has diagnostic importance. When you will make the passive hemagglutination test (PHAT) gut 5 drops of the different solvent of the serum in the hole of the plane-table, then add 1 drop of diagnosticum in each hole.

Ingredients, ml Number of the test wells Controls
0,9% solution of NaCl 0.4 0.4 0.4 0.4 0.4 0.4 0.4 0.4 0.4 -
Assayed serum 1:62.5 0.4 0.4 0.4 0.4 0.4 0.4 0.4 0.4 - -
Diagnosticum 0.1 0.1 0.1 0.1 0.1 0.1 0.1 - 0.1 0.1
Serum dilution 1:125 1:250 1:500 1:1000 1:2000 - - 1:125 - -
Control erythrocytes - - - - - - - 0.1 - -
Standard immune serum, diluted to the titer - - - - - - - - - 0.4

Task 2.

IFT.

R. prowazekii is ovoid shape cells with green shining around their in the picture.

 

 

 


Date: 2016-01-14; view: 470


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