Microbiological diagnosis of the botulism

Serum from patient Urine, Faeces, Foodstuffs

Serological Biological method Bacteriological method

PHAT NT on wite mouse Isolation and identification

the pure culture of the bacteria,

studying type toxin in NT

FINAL RESPONSE

Microbiological diagnosis of the acute intestinal infections, which are caused the non-pathogenic bacteria

Faeces

Bacteriological method

Isolation and identification the pure culture

determination quantity of the esse-pathogenic bacteria

in unit of material for research

FINAL RESPONSE

Microbiological diagnosis of the Staphylococcus aureus food poisoning

Faeces, Vomiting discharges, Foodstuffs, Scourages of stomach

Bacteriological methodExtracted the enterotoxins in

isotonic solution, NT in gel

Grow on EYA for isolation with antitoxic serum A, B, E

and identification

pure culture of the bacteria

Biological method

Study enterotoxigenic Rearing of material to new-born cats

properties in PT in gel with

antitoxic serum A, B, C;

phagotyping test with

the epidemiology object

FINAL RESPONSE

PATOGENESIS FOOD TOXIC INFECTION
↓
Penetration in the digestive canal
(Infected product, pathogen toxins in the product)
↓
(Incubation period 1-12 hours)
↓
Stomach
↓
Some bacteria dies:
nausea, vomiting exhausting (local effect of endotoxin)
general intoxication of the body (generalized effect of endotoxin)
↓
Small Intestine
↓
Effects of pathogen and the mucous membrane enterotoxin
exhausting vomiting
Violation of electrolytic homeostasis - watery diarrhea and vomiting
(Increase cAMP)
Inflammation of the mucous membranes (the action of histamine, serotonine)
↓
congestion, swelling of the mucosa
hemorrhages in the mucous membrane of small and large intestines
The formation of erosions and ulcers (C. perfringens)
absorption of toxins in the blood
Changes in nerve function and blood circulation
↓
In difficult course:
dehydration
Violations of hemodynamics
hypoxia
metabolic acidosis
infectious-toxic shock with lethal outcome

Date: 2016-01-14; view: 1363