ROLE OF CARBOHYDRATE-DEFICIENT TRANSFERRIN IN THE DIAGNOSIS OF ALCOHOLIC INTOXICATION

A.I. PAVLOV, S.A. BELYAKIN

FSI “3^rd Central Military Clinical Hospital named after A.A. Vishnevsky of the Ministry of Defense of Russia; Krasnogorsk, RUSSIA

The main reason of the development of alcoholic hepatic disease (AHD) - Chronic alcohol intoxication.

The aim of the study: To establish appropriateness of the determination of carbohydrate-deficient transferrin for the diagnosis of alcohol intoxication.

Materials and methods: We examined 140 patients (divided into three groups) that took toxic doses of alcohol, to determine the level of carbohydrate-deficient transferrin - a marker of chronic alcohol intoxication. 56 (40.0%) of first group examined for acialoglycosidic transferrin. 37 (26.4%) patients for the second group for - a - mono-and disialotransferrin. The third group - 47 (33.6%) people – for the whole spectrum of cialoglycosidic transferrin deficit: deficit of a-, mono-, di-and tri-sialotransferrin.

Results: Our studies showed that in the identification of only acialtransferrin positive result was found in only 3 (5.4%) patients, admitted to the hospital with severe manifestations of acute alcoholic hepatitis. In identification of the total transferrin deficit, pathological changes were found in 36 (76.6%) patients. This confirms relatively high diagnostic ability in the testing of chronic alcohol intoxication.

Conclusion: Alcohol intoxication plays an important and sometimes fatal role in the progression of alcoholic hepatic disease. Intensive searches and improvements in its diagnosis are carried out. The method of determining carbohydrate-deficient transferrin - a marker of chronic alcohol intoxication was introduced into clinical practice.

The data obtained by us, confirm the literature data that identification of cialoglycosidic transferrin is specific but less informative, however identification of the total transferring deficiency was highly informative method of diagnosis of chronic alcohol intoxication. Identification of tricialoglycosidic transferrin increases diagnostic value of carbohydrate-deficiency test.

ASSESSMENT OF HEPATOPROTECTIVE ACTIVITY OF SODIUM SUCCINATE MEGLUMINE IN THE EXPERIMENTAL INJURIES OF LIVER

A.Y.PETROV, V.A.ZAPLUTANOV, S.V.OKOVITIY, M.G.ROMANTSOV, A.L.KOVALENKO

Russian Military Medical Academy named after S.M. Kirov;

Science and technology pharmaceutical company “POLYSAN”, St. Petersburg, RUSSIA

The number of toxic injuries grows from year to year, and therefore, there is a need for new, more effective means of detoxifying. Hypoxia is a leading syndrome of intoxication by xenobiotics, therefore, an important aspect of drug therapy of hepatic pathological conditions is a correction of the energy balance of the hepatocytes.

Purpose. The study of hepatoprotective properties of sodium succinate meglumine (SSM) in experimental models.

Materials and methods. The study of hepatoprotective activity of Reamberin, solution for infusions (1.5% solution of SSM) was carried out on Wistar rats. As a comparative drug there was used mafusol, solution for infusion. The first group of animals were intraperitoneally injected ammonium chloride, 125 mg / kg (1 / 3 LD₃₀) of 0.9% sodium chloride solution within 7 days. The second group ammonium chloride was injected similarly in 2 ml Reamberin. In the third group ammonium chloride was injected in 2 ml mafusole. The degree of hepatic injury was assessed by the level of intracellular metabolites - pyruvate, phosphoenolpyruvate, phosphocreatine, ATP, and POL products - malondialdehyde. The results are processed using Student's t-test and Fisher’s chi-square.

Results and discussion. Ammonium chloride intoxication is accompanied by a decrease in body weight and increase in relative liver weight. Treatment by Reamberin fully normalized these rates, and application of comparative drug was less effective. On the recovery of homeostasis of hepatocytes, in the application of Reamberin shows normal levels of glycogen, phosphoenolpyruvate, phosphocreatine and ATP (antihypoxia activity). Membrane-stabilizing action of Reamberin and its antioxidant activity is shown by a significant reduction of malondialdehyde in liver cells, as compared with mafusole - 3,2 ± 0,4 and 4,9 ± 0,9 nmol / g, respectively (normal 2,2 ± 0 , 7 nmol / g).

Conclusion. The results shows the effectiveness of meglumine sodium succinate (Reamberin), with hepatoprotective activity due to antihypoxia and membrane stabilyzing effects, exceeding the activity of the drug as compared to the critical indicators of hepatocyte functions, indicating the possibility of application of Reamberin in the clinic for chronic liver damage.

Date: 2014-12-28; view: 745