DISEASES OF ESOPHAGUS, STOMACH and DUODENUM

CHANGE OF MINERAL DENSITY OF BONE TISSUES IN PATIENTS WITH INFLAMMATORY DISEASES OF THE COLON

R.A. ABDULKHAKOV, E.T. KELESIDU, R.V. BOLOTINA

Kazan State Medical University, Kazan, RUSSIA

Purpose: To determine mineral density of bone tissue in patients with inflammatory diseases of the colon.

Materials and methods: A survey of mineral density of the radial and ulnar bones by X-ray densitometry method in doubleabsorbtional osteodensitometry “Osteometer DTX-200” was carried out in 27 patients (m-13 w-14) aged between 20 - 73 years with inflammatory colonic diseases (non-specific ulcerative colitis and Crohn's disease). Duration of disease ranged between one - 29 years. Most patients had chronic recurrent course of the disease, in 7 patients the course of the disease was easy, in 12 – mild, in 8 - severe.

Results: The reduction of mineral density of bone tissue was detected in 8 (61.5%) males and 7 (50%) women. No dependence on sex, age, duration of disease was revealed. There was revealed a tendency to the dependence of the reduction of mineral density of bone on the severity of the underlying disease.

The prevalence of clarythromycin-related A2143G, A2142G and T2717C mutations of H.pylori-23S rRNA in Kazan (Russia)

E.R. ABUZAROVA, R.A. ABDULKHAKOV, V.M. CHERNOV,

O.A. CHERNOVA, S.R. ABDULKHAKOV

Kazan State Medical University, Institute of Biochemistry and Biophysics, Kazan, RUSSIA

Introduction. In Helicobacter pylori (H.pylori) infection resistance to clarythromycin is mostly due to the presence of A2143G, A2142G and T2717C point mutations of the 23S rRNA gene. The aim of our work was to investigate the prevalence of clarythromycin-related mutations of Н.pylori strains among patients with gastroduodenal diseases in Kazan (Russia).

Materials and methods: Gastric biopsies obtained from 86 patients with peptic ulcer disease, chronic gastritis and GERD were examined. H.pylori was revealed by cytology, rapid urease test and ureC PCR analysis (“Lytech”, Russia). H.pylori-positive biopsies were taken for further evaluation. To detect A2142G, A2143G and T2717C mutations of the 23S rRNA gene MboII-, Bso31I (BsaI)- and AspLE (HhaI)-restriction PCR-RFLP assays were conducted (“SibEnzim”, Russia).

Results: As a result of cytology, rapid urease tests and PCR analysis, H.pylori was revealed in 70 samples. A2143G mutations determining clarythromycin resistance were revealed in 8 out of 70 (11.4%) examined gastric biopsies. A2142G and T2717C mutations weren’t found in any biopsies.

Conclusions: It was found that the prevalence of clarythromycin-resistant H.pylori strains is 11.4% (8/70) among patients with gastroduodenal pathology in Kazan (Russia). These numbers (11.4%) of clarythromycin resistance allow to start eradication with standard first-line therapy consisting of PPI, amoxicillin and clarythromycin.

Date: 2014-12-28; view: 867