EFFECTIVENESS OF PHOTODYNAMIC THERAPY (PDT) IN EXPERIMENTAL PERITONITIS

A.V. GEYNITS, R.D. MUSTAFAYEV, R.I. KIZEVADZE, G.V. TIKHOV

State Research and Clinical Center for Laser Medicine, Moscow, RUSSIA

Purpose: To evaluate the effectiveness of photodynamic therapy (PDT) for treating generalized peritonitis.

Materials and methods: 68 rats of Wistar line weighing 200-250 gr were used in the present study. To create the model of acute diffuse fecal peritonitis a modified technique (Lazarenko et al) was used: 10% filtrated fecal suspension in the dosage 0.5 ml per 100 gr. After inoculation of the abdominal cavity with fecal suspension a clinical picture of acute peritonitis in experimental animals developed on the third day. The animals were flaccid and inactive; they had bloated abdomen, no appetite and no stool. To assess the effectiveness of treatment of diffuse peritonitis experimental animals were divided into two groups- main and control (22 animals). In the control group the mostly common technique for abdomen sanation was used: 0.02% chlorhexidine solution. Besides, antibacterial therapy with Gentamicin ( 2 mg per kg of body weight intravenously) was made for three postoperative days. In the main group there were 43 rats. For the sanation of their abdominal cavity PDT technique was used. Experimental animals had an i/v injection with photosensitizer Photoditazine in dosage 0.8 mg/kg. 2 – 2.5 hours later – as it was thought to be the time of maximal photosensitizer accumulation in the inflamed abdominal cavity – the animals were operated on. Laser apparatus “Atkus-2” with output power 1-2Wt, continuous mode, red optic range, wavelength 661 nm was used for PDT session.

Results: Mortality rate in the main group was 9.5% ( 4 rats). Two rats died within 24 hours and two other rats died within 48 hours because of continuing peritonitis. In the control group mortality rate was 27.3% (6 rats ). The animals died within the first 24 hours having symptoms of continuing peritonitis.

Conclusion: The obtained mortality rate in experimental peritonitis indicates that PDT used for the sanation of the abdominal cavity is more effective than only a traditional technique.

Date: 2014-12-28; view: 916